Applicable for use in assessing the capacity of T cells to maintain metabolic fitness in metabolically restrictive conditions (e.g., tumor microenvironment).Suitable for evaluation of different construct designs, engineering strategies, starting material selection, or metabolic conditioning during the cell expansion process.Provide a comprehensive view of T cell energy metabolism.Supports two assays for generating robust bioenergetic parameters linked to critical attributes for antitumor properties: T cell persistence and T cell metabolic fitness.The XFp kit is for use with the Agilent Seahorse HS Mini and XFp analyzers.
Tcel journal pro#
The XF kit is for use with Agilent Seahorse XF Pro and XFe/XF96 analyzers. The XF T Cell Metabolic Profiling kit is also integrated with Wave Pro and Seahorse Analytics software to simplify data analysis, visualization, and interpretation.Įach assay kit contains sufficient materials for six full-plate tests and is available in two packaging sizes.
The kit not only provides improved reagents, but also features a streamlined assay workflow, reducing assay preparation time and minimizing the need for uncoupler optimization. These measurements can be linked to antitumor properties of T cell therapy products and are therefore valuable in designing and optimizing therapy development processes to improve T cell persistence or avoid exhaustion in the tumor microenvironment. The Agilent Seahorse XF T Cell Metabolic Profiling kit allows for robust and accurate measurements of both glycolytic and mitochondrial activities in T cell populations, providing a complete picture of T cell energy metabolism.
Tcel journal full#
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